Journal: Cell reports

Article Title: Targeting EP2 receptor with multifaceted mechanisms for high-risk neuroblastoma

doi: 10.1016/j.celrep.2022.111000

Figure Lengend Snippet: (A) Human NB cell lines (SK-N-AS, SK-N-SH, SH-SY5Y, CHLA-90, NB-EBc1, SK-N-BE(2), BE(2)-C, CHLA-136, SiMa, IMR-32, NB-1691), human fibroblast cell line Hs68, and mouse NB cell lines (Neuro-2a and NXS2) were treated with PGE 2 (10 μM), selective EP2 agonist butaprost (10 μM), EP4 agonist CAY10598 (10 μM), or forskolin (100 μM) as positive control. The cAMP signaling in these cells was detected by a TR-FRET method (n = 4–6, *p < 0.05; **p < 0.01; ***p < 0.001, compared with the control group, one-way ANOVA and post hoc Dunnett’s test). Data are presented as mean + SEM. (B) The cAMP production via EP2 receptor activation by butaprost and EP4 activation by CAY10598 in all 13 NB cell lines was compared (p < 0.0001, two-tailed paired t test).

Article Snippet: Human NB cell lines SK-N-AS (sex: female), SK-N-SH (sex: female), SH-SY5Y(sex: female), SK-N-BE(2) (sex: male), BE(2)-C (sex: male), IMR-32 (sex: male), mouse NB cell line Neuro-2a (sex: male), and human normal fibroblast cell line Hs68 (sex: male) from the American Type Culture Collection (ATCC) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, HyClone) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Gibco).

Techniques: Positive Control, Control, Activation Assay, Two Tailed Test

Journal: Cell reports

Article Title: Targeting EP2 receptor with multifaceted mechanisms for high-risk neuroblastoma

doi: 10.1016/j.celrep.2022.111000

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human NB cell lines SK-N-AS (sex: female), SK-N-SH (sex: female), SH-SY5Y(sex: female), SK-N-BE(2) (sex: male), BE(2)-C (sex: male), IMR-32 (sex: male), mouse NB cell line Neuro-2a (sex: male), and human normal fibroblast cell line Hs68 (sex: male) from the American Type Culture Collection (ATCC) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, HyClone) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Gibco).

Techniques: Virus, Recombinant, Modification, SYBR Green Assay, CRISPR, Expressing, Plasmid Preparation, shRNA, Software, Fluorescence, Microscopy, Spectrophotometry

Journal: Stem cells (Dayton, Ohio)

Article Title: Multipotent Stromal Cells (MSCs) are Activated to Reduce Apoptosis in Part by Upregulation and Secretion of Stanniocalcin-1 (STC-1)

doi: 10.1002/stem.20080742

Figure Lengend Snippet: Table 1

Article Snippet: For the co-culture experiments with irradiated fibroblasts, normal human diploid dermal skin fibroblasts (HS-68; American Tissue Type Culture Collection (ATCC), Rockville, MD) were thawed and plated at 10,000 cells/cm 2 on a 4.6 cm 2 transwell inserts (pore size 0.4 μm, #3450; Corning, New York, NY) in 2 mL growth medium (low glucose DMEM (GIBCO/BRL), 10% FBS and 100 units/mL penicillin).

Techniques: Cell Culture, Irradiation

Journal: Oncotarget

Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity

doi: 10.18632/oncotarget.6488

Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Article Snippet: The normal human foreskin fibroblast cell line Hs68 was provided by JCRB Cell Bank (Osaka, Japan).

Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy